5) Why pipetting training is crucial for clinical technician?
By having a proper pipette training, always helps to minimize the risk of volume variability caused by Operators, also a small fraction of the change in pipetting can give you the wrong result.
An aliquot is the known amount of homogeneous material, used to minimize the sampling error. It is usually used when fractional part is an exact divisor of the whole.
9) Explain what is the difference between sterilization and disinfection?
Sterilization: The thorough sterilization of all microbes present on the surgical instrument is referred as Sterilization
Disinfection: While reducing the total number of microbes below the risk level is referred as Disinfection
12) Explain what clinical lab audit is and what are the areas you can do clinical audit?
A clinical lab audit is done in order to maintain and operate the lab at a standard level. The area that includes in clinical audit are
Specimens: To check the patient register and see whether the specimen was received at the right time
Turnaround time: To check whether the specimen was tested and returned at allocated time, and if delayed how to improve it
GLP: To check whether the test methods carried out follows the standard procedures
Purchasing equipment’s, reagents and other lab instruments
Laboratory reports: To check whether they are precise and clear and look for any area for improvement
Storage of reagents and specimens
Safety policies and procedures: Use of dangerous substances should be audited, and every single accident in the lab should be recorded.
Previous Companies:
Accu Reference Medical Lab
Langton Green Inc
Medstar Southern Md Hospital
7) What are the different techniques for placing samples in micro-scope?
Different techniques used for placing samples under micro-scope are
Dry Mount: You simply put section of specimen with a cover slip over a sample
Wet Mount: Samples are placed under various liquid medium like glycerine, water, brine and water
Smear Slides: In this technique, sample is smear over the slide and on top it another slide is placed without forming bubbles
Squash Slides: In this technique, lens tissue is used over the wet mount, and it will remove excess water
Staining: Stains such as iodine, methylene blue and crystal violet is used to stain the specimen
13) Explain what is laboratory centrifuges?
Laboratory Centrifuge is primarily used for testing liquids and substances for clinical trial samples. This device uses the centrifugal force to separate the liquids from the main sample or mixture.
When sample is rotated into centrifuge, it will separate the mixture according to the density. Supernatant is the upper layer found in the sample after it is run into centrifuge.
15) What are the steps you can take to avoid imbalance in centrifuge?
To avoid an imbalance in centrifuge it requires
Balanced loading of the centrifuge rotor
Even number of tubes should be loaded facing each other or in the opposite direction
When odd number of tubes are loaded make sure, you make it even with adding one more tube with an equal amount of water of that of the sample tube
Blank term is used to refer the sample tube which does not contain the analyte.
19) What do you mean by a confirmatory test?
For unambiguous identification of drug or metabolites in the sample, alternative chemical method is used also known as a second test.
11) What are the factors on which the gas sterilization depends on?